@

Clone-Picking
Nylon Filter

Custom Standard cDNA Library Construction

AGCTs innovative technology combines optimized cDNA synthesis and ligation methods to maximize quality of libraries.

The size of starting materials determines the form of the library: for samples greater than 0.5 g of tissue or 1 mg total RNA, libraries can be built in unamplified procedure.  In the pBluescript II and lambda phage vectors, the library properties are:

Primary clones:  2 X 106 cfu

Insert range:  0.5 - 3 kb

% recombinants:  90 - 95%

Turn around time: 

pBluescriptII vector:  2 - 3 weeks

lambda phage vector (w/ amplification):  3 - 4 weeks

For 25 - 50 ng mRNA samples, libraries will be built in amplified procedure in the pDNAscript vector.  The library properties are:

Primary clones:  1 X 107 cfu

Insert range:  0.5 - 2 kb

% recombinants:  95%

Turn around time:  2 - 3 weeks

Code Description Price
cDNACon cDNA Library Construction please inquire
QC24 QC by PCR 24 clones free - $80
QC-96 QC 96 Sequencing $800.00
QC-E QC - by Enzyme Cutting free - $250
N-f Nylon Filter per sheet $500.00 - 600.00
Pick Clone Picking $0.25 per clone