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Clone-Picking
Nylon Filter

Custom Full Length cDNA Library Construction

AGCT's Full Length cDNA Library Construction Service is the only one of its kind in the world, utilizing innovative methods to construct high-quality, truly full-length libraries.  Our scientists have developed an improved procedure for the purification and ligation of only the dscDNA with 5' end Cap and 3' end poly A tail molecules into a suitable vector.  Theoretically, the library built from our kit would contain 100% full length genes.  AGCT guarantees that the Full Length cDNA Library we construct for you will contain at least 95% full length genes.

The minimum size of starting materials must be 1 mg of total RNA, 1 g of tissue or 106 cells.


Highlights:

-  Only full length dscDNA molecules used, allowing for the creation of true full-length cDNA libraries;

-  New, faster, more efficient procedures;

-  No need for PRC amplification, allowing for large, high-representational libraries;

-  Flexibility in host vector choices;

-  Directional cloning, perfect for down stream gene expression studies;

-  3 - 4 weeks turn around time.


Library properties are:

- % full-length genes:  95%

- Primary clones:  2 X 106 cfu

- Inserts range:  0.5 V 3 kb

- % recombinants:  95%

- Turn around time:  3 V 4 weeks

- Q.C. by PCR:  P.C.R 24 random colonies

Code Description Price
FLcDNA FL cDNA Library Construction please inquire
QC24 QC by PCR 24 clones $80
QC-96 QC 96 Sequencing $800.00
QC-E QC - by Enzyme Cutting $250
N-f Nylon Filter per sheet $500.00 - $600.00
Pick Clone Picking $0.22 per clone